OXCT1 anticorps (AA 35-309)

N° du produit ABIN7601419

L’anticorps Lapin Polyclonal anti-OXCT1 a été validé pour WB, ELISA, IF, ICC et FACS. Il convient pour détecter OXCT1 dans des échantillons de Humain, Souris, Rat et Singe.

Aperçu rapide pour OXCT1 anticorps (AA 35-309) (ABIN7601419)

Antigène: OXCT1 (3-Oxoacid CoA Transferase 1 (OXCT1))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp OXCT1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-OXCT1 anticorps

Épitope: AA 35-309

Fonction: Anti-OXCT1/SCOT Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-OXCT1/SCOT Antibody Picoband® (ABIN7601419). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human OXCT1/SCOT recombinant protein (Position: S35-A309).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fukao, T., Mitchell, G. A., Song, X.-Q., Nakamura, H., Kassovska-Bratinova, S., Orii, K. E., Wraith, J. E., Besley, G., Wanders, R. J. A., Niezen-Koning, K. E., Berry, G. T., Palmieri, M., Kondo, N. Succinyl-CoA:3-ketoacid CoA transferase (SCOT): cloning of the human SCOT gene, tertiary structural modeling of the human SCOT monomer, and characterization of three pathogenic mutations. Genomics 68: 144-151, 2000. 2. Hori, T., Fukao, T., Murase, K., Sakaguchi, N., Harding, C. O., Kondo, N. Molecular basis of two-exon skipping (exons 12 and 13) by c.1248+5g-a in OXCT1 gene: study on intermediates of OXCT1 transcripts in fibroblasts. Hum. Mutat. 34: 473-480, 2013. 3. Kassovska-Bratinova, S., Fukao, T., Song, X.-Q., Duncan, A. M. V., Chen, H. S., Robert, M.-F., Perez-Cerda, C., Ugarte, M., Chartrand, C., Vobecky, S., Kondo, N., Mitchell, G. A. Succinyl CoA:3-oxoacid CoA transferase (SCOT): human cDNA cloning, human chromosomal mapping to 5p13, and mutation detection in a SCOT-deficient patient. Am. J. Hum. Genet. 59: 519-528, 1996.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur OXCT1

Antigène: OXCT1 (3-Oxoacid CoA Transferase 1 (OXCT1))

Autre désignation: OXCT1

Sujet:

Synonyms: DNA- (apurinic or apyrimidinic site) lyase 2,3.1.-.-,4.2.99.18,AP endonuclease XTH2,APEX nuclease 2,APEX nuclease-like 2,Apurinic-apyrimidinic endonuclease 2,AP endonuclease 2,APEX2,APE2, APEXL2, XTH2,

Tissue Specificity: Highly expressed in brain and kidney. Weakly expressed in the fetal brain. .

Background: 3-oxoacid CoA-transferase 1 (OXCT1) is an enzyme that in humans is encoded by the OXCT1 gene. It is also known as succinyl-CoA-3-oxaloacid CoA transferase (SCOT). This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency.

Poids moléculaire: 56 kDa

ID gène: 5019

UniProt: P55809

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis